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Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime

Blacker, TS; Berecz, T; Duchen, MR; Szabadkai, G; (2017) Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime. Bio Protocol , 7 (2) , Article e2105. 10.21769/BioProtoc.2105. Green open access

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Abstract

NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their spectrally-identical autofluorescence, termed NAD(P)H, can be used to probe the mitochondrial redox state, while their distinct enzyme-binding characteristics can be used to separate their relative contributions to the total NAD(P)H intensity using fluorescence lifetime imaging microscopy (FLIM). These protocols allow differences in metabolism to be detected between cell types and altered physiological and pathological states.

Type: Article
Title: Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.21769/BioProtoc.2105
Publisher version: http://doi.org/10.21769/BioProtoc.2105
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Autofluorescence, FLIM, Fluorescence lifetime, Microscopy, NAD(P)H, NADH, NADPH, Redox state
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: http://discovery.ucl.ac.uk/id/eprint/1546850
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