UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Experimental and in silico computational studies of novel nanoparticle vaccine adjuvants

Maughan, CN; (2017) Experimental and in silico computational studies of novel nanoparticle vaccine adjuvants. Doctoral thesis , UCL (University College London).

[img] Text
Maughan_Thesis_CharlotteMaughan_UCLPhDAward.pdf
Access restricted to UCL open access staff until 1 May 2018.

Download (18MB)

Abstract

This thesis applies inorganic chemistry to develop new nanoparticulate vaccine adjuvants, entities added to a vaccine to inculcate robust immunity. The Introduction sets out the background to this work, and the Experimental Methods chapter reviews the techniques applied. Chapter 3 then reports the preparation of AlO(OH) using a continuous hydrothermal synthetic pathway – with sub-100 °C temperatures and atmospheric pressure – to produce nanoscale particles. A variety of experimental parameters was explored, and some degree of particle engineering could be achieved albeit over a narrow range of sizes and shapes. There was some evidence of particle size influencing the response of macrophages to the samples. Chapter 4 and Chapter 5 aim to engineer the size and shape of hydroxyapatite and zinc oxide nanoparticles, respectively, through different continuous hydrothermal processes. Several different morphologies (spheres, and mixtures of spheres/rods/platelets) could be produced. The morphology and particle size appear to affect cytokine production in vitro. Chapter 6 explores layered double hydroxides (LDHs) as inorganic adjuvants. A series of materials were prepared and characterised, and the effect of changing the LDH chemical composition on adjuvanticity determined. It was found that the size of the guest anion influences the immune response. Further, computational models were developed to aid the in silico prediction of immunogenicity, with calculated energy values being a suitable proxy for the zeta potential. The related hydroxy double salt (HDS) materials are investigated as adjuvants in Chapter 7. A series of materials was prepared and their chemical composition found to markedly effect the immune response in vitro. Computational models were sought with the same in silico aim as the LDH materials, however with limited success owing to a lack of detailed structural knowledge in the literature. Finally, overarching conclusions and suggestions for the future outlook of this area of research are given in Chapter 8.

Type: Thesis (Doctoral)
Title: Experimental and in silico computational studies of novel nanoparticle vaccine adjuvants
Event: UCL (University College London)
Language: English
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: http://discovery.ucl.ac.uk/id/eprint/1546603
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item