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Targeted inactivation of Fh1 causes proliferative renal cyst development and activation of the hypoxia pathway

Pollard, PJ; Spencer-Dene, B; Shukla, D; Howarth, K; Nye, E; El-Bahrawy, M; ... Tomlinson, IPM; + view all (2007) Targeted inactivation of Fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. CANCER CELL , 11 (4) 311 - 319. 10.1016/j.ccr.2007.02.005.

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Abstract

Germline mutations in the fumarate hydratase (FM tumor suppressor gene predispose to leiomyomatosis, renal cysts, and renal cell cancer (HLRCC). HLRCC tumors overexpress HIR1 alpha and hypoxia pathway genes. We conditionally inactivated mouse Fh1 in the kidney. Fh1 mutants developed multiple clonal renal cysts that overexpressed Hif1 alpha and Hif2 alpha. Hif targets, such as Glut1 and Vegf, were upregulated. We found that Fh1-deficient murine embryonic stem cells and renal carcinomas from HLRCC showed similar overexpression of HIF and hypoxia pathway components to the mouse cysts. Our data have shown in vivo that pseudohypoxic drive, resulting from HIF1 alpha (and HIF2 alpha) overexpression, is a direct consequence of Fh1 inactivation. Our mouse may be useful for testing therapeutic interventions that target angiogenesis and HIF-prolyl hydroxylation.

Type:Article
Title:Targeted inactivation of Fh1 causes proliferative renal cyst development and activation of the hypoxia pathway
DOI:10.1016/j.ccr.2007.02.005
Keywords:HIPPEL-LINDAU-DISEASE, MITOCHONDRIAL-DNA MUTATIONS, TUMOR-SUPPRESSOR PROTEIN, CELL CANCER, HEREDITARY LEIOMYOMATOSIS, FUMARATE-HYDRATASE, HIF-ALPHA, SUCCINATE-DEHYDROGENASE, 3-NITROPROPIONIC ACID, GENITOURINARY TRACT
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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