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3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds

Bradley, RS; Robinson, IK; Yusuf, M; (2016) 3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds. Macromolecular Bioscience , 17 (2) , Article 1600236. 10.1002/mabi.201600236. Green open access

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Abstract

Here, it is demonstrated that X-ray nanotomography with Zernike phase contrast can be used for 3D imaging of cells grown on electrospun polymer scaffolds. The scaffold fibers and cells are simultaneously imaged, enabling the influence of scaffold architecture on cell location and morphology to be studied. The high resolution enables subcellular details to be revealed. The X-ray imaging conditions were optimized to reduce scan times, making it feasible to scan multiple regions of interest in relatively large samples. An image processing procedure is presented which enables scaffold characteristics and cell location to be quantified. The procedure is demonstrated by comparing the ingrowth of cells after culture for 3 and 6 days.

Type: Article
Title: 3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/mabi.201600236
Publisher version: http://dx.doi.org/10.1002/mabi.201600236
Language: English
Additional information: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: Cell infiltration; electrospun scaffolds; imaging; microstructure; X-ray nanotomography
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1537569
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