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Post-prandial adjustments in renal phosphate excretion do not involve a gut-derived phosphaturic factor

Lee, GJ; Hashimi, L; Debnam, ES; Unwin, RJ; Marks, J; (2017) Post-prandial adjustments in renal phosphate excretion do not involve a gut-derived phosphaturic factor. Experimental Physiology , 102 (4) pp. 462-474. 10.1113/EP086062. Green open access

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Abstract

To date, the role of the small intestine in regulating post-prandial phosphate homeostasis has remained unclear and controversial. Previous studies have proposed the presence of a gut-derived phosphaturic factor that acts independently of changes in plasma phosphate concentration or parathyroid hormone (PTH) level; however, these early studies used duodenal luminal phosphate concentrations in the molar range and therefore the physiological relevance of this is uncertain. In the present study, we used both in vivo and in vitro approaches to investigate the presence of this putative ‘intestinal phosphatonin’. Instillation of 1.3M phosphate into the duodenum rapidly induced phosphaturia, but in contrast to previous reports, this was associated with significant hyperphosphataemia and elevated PTH level; however, there was not the expected decrease in abundance of the renal sodium-phosphate cotransporter NaPi-IIa. Instillation of a physiological (10mM) phosphate load had no effect on plasma phosphate concentration, PTH level or phosphate excretion. Moreover, phosphate uptake by opossum kidney cells was unaffected after incubation with serosal fluid collected from intestinal segments perfused with different phosphate concentrations. Taken together, these findings do not support the concept of a gut-derived phosphaturic factor that can mediate rapid signalling between gut and kidney, leading to increased urinary phosphate excretion, as part of normal phosphate homeostasis.

Type: Article
Title: Post-prandial adjustments in renal phosphate excretion do not involve a gut-derived phosphaturic factor
Open access status: An open access version is available from UCL Discovery
DOI: 10.1113/EP086062
Publisher version: http://doi.org/10.1113/EP086062
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Kidney, intestine, phosphate
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/1536103
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