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MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer

Matull, WR; Andreola, F; Loh, A; Adiguzel, Z; Deheragoda, M; Qureshi, U; ... Pereira, SP; + view all (2008) MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer. BRIT J CANCER , 98 (10) 1675 - 1681. 10.1038/sj.bjc.6604364. Gold open access

Abstract

Alterations in epithelial mucin expression are associated with carcinogenesis, but there are few data in biliary tract cancer (BTC). In pancreatic malignancy, MUC4 is a diagnostic and prognostic tumour marker, whereas MUC5AC has been proposed as a sensitive serological marker for BTC. We assessed MUC4 and MUC5AC expression in (i) prospectively collected bile and serum specimens from 72 patients with biliary obstruction (39 BTC) by real-time reverse transcriptase-PCR (qPCR) and western blot analysis, and (ii) 79 archived biliary tissues (69 BTC) by immunohistochemistry. In bile, MUC4 protein was detected in 27% of BTC and 29% of primary sclerosing cholangitis (PSC) cases, but not in other benign and malignant biliary diseases (P < 0.01 and P 0.06). qPCR revealed a 1.9-fold increased MUC4 mRNA expression in BTC patients' bile compared with benign disease. In archived tissues, MUC4 protein was detected in 37% of BTC but in none of the benign samples (P = 0.03). In serum, MUC5AC was found exclusively in BTC and PSC sera (44% and 13%, respectively; P < 0.001 for BTC vs non-BTC) and correlated negatively with BTC survival. Biliary MUC4 and serum MUC5AC are highly specific tumour-associated mucins that may be useful in the diagnosis and formulation of therapeutic strategies in BTC.

Type:Article
Title:MUC4 and MUC5AC are highly specific tumour-associated mucins in biliary tract cancer
Open access status:An open access publication
DOI:10.1038/sj.bjc.6604364
Publisher version:http://www.ncbi.nlm.nih.gov/pmc/ articles/PMC2391120/?tool=pubmed
Keywords:biliary tract cancer, mucin glycoprotein, tumour marker, cholangiocarcinoma, PROGNOSTIC-FACTOR, MONOCLONAL-ANTIBODIES, PANCREATIC-CANCER, GENE-EXPRESSION, CELL-SURFACE, CHOLANGIOCARCINOMA, DIAGNOSIS, SERUM, INHIBITION, CARCINOMA
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Genetics, Evolution and Environment

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