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Three antibodies to human intestinal brush border membrane are characterized as anti-Le(a).

Dickson, L; Richman, PI; Shaw, MA; Donald, AS; Swallow, DM; (1989) Three antibodies to human intestinal brush border membrane are characterized as anti-Le(a). J Immunogenet , 16 (3) pp. 193-202.

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Abstract

In the course of raising monoclonal antibodies to human colonic and small intestinal epithelium, three were isolated which recognized epitopes on many colonic glycoproteins and many jejunal glycoproteins taken from some individuals, but not on the same glycoproteins from others. The specificities of these antibodies were examined by carrying out inhibitions of the binding of the antibodies to the jejunal glycoproteins by blood group substances and purified oligosaccharides. The glycoproteins were separated by electrophoresis and transferred to nitrocellulose strips which were then used for the inhibition experiments. The results indicated that the antibodies bind to Le(a)-active structures. The conditions were determined for achieving red cell agglutination by two of the antibodies.

Type: Article
Title: Three antibodies to human intestinal brush border membrane are characterized as anti-Le(a).
Location: England
Keywords: Animals, Antibodies, Monoclonal, Binding, Competitive, Carbohydrate Sequence, Colon, Epitopes, Glycoproteins, Humans, Immunization, Immunoblotting, Intestinal Mucosa, Lewis Blood-Group System, Mice, Mice, Inbred BALB C, Microvilli, Molecular Sequence Data
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/1527653
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