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Nanoscopic compartmentalization of membrane protein motion at the axon initial segment

Albrecht, D; Winterflood, CM; Sadeghi, M; Tschager, T; Noé, F; Ewers, H; (2016) Nanoscopic compartmentalization of membrane protein motion at the axon initial segment. The Journal of Cell Biology , 215 (1) pp. 37-46. 10.1083/jcb.201603108. Green open access

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Abstract

The axon initial segment (AIS) is enriched in specific adaptor, cytoskeletal, and transmembrane molecules. During AIS establishment, a membrane diffusion barrier is formed between the axonal and somatodendritic domains. Recently, an axonal periodic pattern of actin, spectrin, and ankyrin forming 190-nm-spaced, ring-like structures has been discovered. However, whether this structure is related to the diffusion barrier function is unclear. Here, we performed single-particle tracking time-course experiments on hippocampal neurons during AIS development. We analyzed the mobility of lipid-anchored molecules by high-speed single-particle tracking and correlated positions of membrane molecules with the nanoscopic organization of the AIS cytoskeleton. We observe a strong reduction in mobility early in AIS development. Membrane protein motion in the AIS plasma membrane is confined to a repetitive pattern of ∼190-nm-spaced segments along the AIS axis as early as day in vitro 4, and this pattern alternates with actin rings. Mathematical modeling shows that diffusion barriers between the segments significantly reduce lateral diffusion along the axon.

Type: Article
Title: Nanoscopic compartmentalization of membrane protein motion at the axon initial segment
Open access status: An open access version is available from UCL Discovery
DOI: 10.1083/jcb.201603108
Publisher version: http://dx.doi.org/10.1083/jcb.201603108
Language: English
Additional information: Copyright © 2016 Albrecht et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1527100
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