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OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response.

Farashahi Yazd, E; Rafiee, MR; Soleimani, M; Tavallaei, M; Salmani, MK; Mowla, SJ; (2011) OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response. Cancer Lett , 309 (2) pp. 170-175. 10.1016/j.canlet.2011.05.027.

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Abstract

A novel variant of OCT4, OCT4B1, is highly expressed in pluripotent cells. OCT4B1 is also upregulated in various tumors and cell lines. Here, we have constructed a HA-tagged OCT4B1 construct, which could be translated into an N-terminally tagged protein, detectable by HA antibody. Western blotting revealed that OCT4B1 can act as a precursor for OCT4B, and it can also produce a truncated protein with a molecular size corresponding to that of predicted OCT4B1. Furthermore, our data demonstrated a cytoplasmic distribution for both isoforms. Interestingly, the expression ratio of OCT4B1/OCT4B transcripts and proteins was significantly elevated under the heat-stress condition.

Type: Article
Title: OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response.
Location: Ireland
DOI: 10.1016/j.canlet.2011.05.027
Keywords: Alternative Splicing, Biomarkers, Blotting, Western, Cell Line, HEK293 Cells, Heat-Shock Response, Humans, Immunohistochemistry, Octamer Transcription Factor-3, Pluripotent Stem Cells, Protein Isoforms, RNA Splicing, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Stress, Physiological
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/1524619
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