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Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis

Jeffries, JWE; Dawson, N; Orengo, C; Moody, TS; Quinn, DJ; Hailes, HC; Ward, JM; (2016) Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis. ChemistrySelect , 1 (10) pp. 2217-2220. 10.1002/slct.201600515. Green open access

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Abstract

Biocatalytic reactions are increasingly being used as a sustainable strategy in organic synthesis and it is recognised that there is need for new enzyme discovery. To establish the utility and versatility of a metagenomics approach, metagenomic DNA extracted from the oral cavity was sequenced and used to create an in silico contig library. This enables individual open reading frames, operons or all the enzymes of a particular family to be identified and then retrieved from the original DNA by PCR. As proof of principle a lactate dehydrogenase, a malate dehydrogenase and transketolases were identified in silico, successfully cloned and assayed. This new enzyme retrieval sequence directed method gives constructive access to metagenomic diversity and importantly improves on the low hit rate experienced when using conventional metagenomic screens.

Type: Article
Title: Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/slct.201600515
Publisher version: http://doi.org/10.1002/slct.201600515
Language: English
Additional information: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Jeffries, JWE; Dawson, N; Orengo, C; Moody, TS; Quinn, DJ; Hailes, HC; Ward, JM; (2016) Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis. ChemistrySelect , 1 (10) pp. 2217-2220. 10.1002/slct.201600515, which has been published in final form at http://doi.org/10.1002/slct.201600515. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: http://discovery.ucl.ac.uk/id/eprint/1524199
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