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Random-access scanning microscopy for 3D imaging in awake behaving animals

Nadella, KM; Roš, H; Baragli, C; Griffiths, VA; Konstantinou, G; Koimtzis, T; Evans, GJ; ... Silver, RA; + view all (2016) Random-access scanning microscopy for 3D imaging in awake behaving animals. Nature Methods , 13 (12) pp. 1001-1004. 10.1038/nmeth.4033. Green open access

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Abstract

Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal dendrites of neocortical pyramidal cells in awake behaving mice.

Type: Article
Title: Random-access scanning microscopy for 3D imaging in awake behaving animals
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/nmeth.4033
Publisher version: http://dx.doi.org/10.1038/nmeth.4033
Language: English
Additional information: Copyright © 2016 Nature America, Inc. All rights reserved.
Keywords: Ca2+ imaging; Cellular neuroscience; Fluorescence imaging; Mouse; Sensorimotor processing
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1522589
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