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Organelle size control – increasing vacuole content activates SNAREs to augment organelle volume through homotypic fusion

Desfougères, Y; Neumann, H; Mayer, A; (2016) Organelle size control – increasing vacuole content activates SNAREs to augment organelle volume through homotypic fusion. J Cell Sci , 129 pp. 2817-2828. 10.1242/jcs.184382. Green open access

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Abstract

Cells control the size of their compartments relative to cell volume, but there is also size control within each organelle. Yeast vacuoles neither burst nor do they collapse into a ruffled morphology, indicating that the volume of the organellar envelope is adjusted to the amount of content. It is poorly understood how this adjustment is achieved. We show that the accumulating content of yeast vacuoles activates fusion of other vacuoles, thus increasing the volume-to-surface ratio. Synthesis of the dominant compound stored inside vacuoles, polyphosphate, stimulates binding of the chaperone Sec18/NSF to vacuolar SNAREs, which activates them and triggers fusion. SNAREs can only be activated by lumenal, not cytosolic, polyphosphate (polyP). Control of lumenal polyP over SNARE activation in the cytosol requires the cytosolic cyclin-dependent kinase Pho80-Pho85 and the R-SNARE Nyv1. These results suggest that cells can adapt the volume of vacuoles to their content through feedback from the vacuole lumen to the SNAREs on the cytosolic surface of the organelle.

Type: Article
Title: Organelle size control – increasing vacuole content activates SNAREs to augment organelle volume through homotypic fusion
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.184382
Publisher version: http://dx.doi.org/10.1242/jcs.184382
Language: English
Additional information: © 2016. Published by The Company of Biologists Ltd. The final publication is available at The Royal Society via http://dx.doi.org/10.1242/jcs.184382.
Keywords: Lysosome, Membrane fusion, Organelle size, Polyphosphate, SNAREs, Vacuole
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1521430
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