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Rapid Formation of Cell Aggregates and Spheroids Induced by a "Smart" Boronic Acid Copolymer

Amaral, AJR; Pasparakis, G; (2016) Rapid Formation of Cell Aggregates and Spheroids Induced by a "Smart" Boronic Acid Copolymer. ACS Applied Materials & Interfaces , 8 (35) pp. 22930-22941. 10.1021/acsami.6b07911. Green open access

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Abstract

Cell surface engineering has emerged as a powerful approach to forming cell aggregates/spheroids and cell–biomaterial ensembles with significant uses in tissue engineering and cell therapeutics. Herein, we demonstrate that cell membrane remodeling with a thermoresponsive boronic acid copolymer induces the rapid formation of spheroids using either cancer or cardiac cell lines under conventional cell culture conditions at minute concentrations. It is shown that the formation of well-defined spheroids is accelerated by at least 24 h compared to non-polymer-treated controls, and, more importantly, the polymer allows for fine control of the aggregation kinetics owing to its stimulus response to temperature and glucose content. On the basis of its simplicity and effectiveness to promote cellular aggregation, this platform holds promise in three-dimensional tissue/tumor modeling and tissue engineering applications.

Type: Article
Title: Rapid Formation of Cell Aggregates and Spheroids Induced by a "Smart" Boronic Acid Copolymer
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acsami.6b07911
Publisher version: http://dx.doi.org/10.1021/acsami.6b07911
Language: English
Additional information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see: http://dx.doi.org/10.1021/acsami.6b07911.
Keywords: Science & Technology, Technology, Nanoscience & Nanotechnology, Materials Science, Multidisciplinary, Science & Technology - Other Topics, Materials Science, cell spheroids, 3D cell culture, cell surface engineering, cell therapy, thermoresponsive polymer, PNIPAAm, boronic acid, tissue engineering, Multicellular Tumor Spheroids, Critical Solution Temperature, Cancer-cells, N-isopropylacrylamide, Surface Modification, Drug-delivery, Culture, 3d, Tissue, Generation
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: http://discovery.ucl.ac.uk/id/eprint/1515906
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