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Effective assembly of fimbriae in Escherichia coli depends on the translocation assembly module nanomachine

Stubenrauch, C; Belousoff, MJ; Hay, ID; Shen, H-H; Lillington, J; Tuck, KL; Peters, KM; ... Lithgow, T; + view all (2016) Effective assembly of fimbriae in Escherichia coli depends on the translocation assembly module nanomachine. Nature Microbiology , 1 , Article 16064. 10.1038/NMICROBIOL.2016.64. Green open access

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Abstract

Outer membrane proteins are essential for Gram-negative bacteria to rapidly adapt to changes in their environment. Intricate remodelling of the outer membrane proteome is critical for bacterial pathogens to survive environmental changes, such as entry into host tissues1,​2,​3. Fimbriae (also known as pili) are appendages that extend up to 2 μm beyond the cell surface to function in adhesion for bacterial pathogens, and are critical for virulence. The best-studied examples of fimbriae are the type 1 and P fimbriae of uropathogenic Escherichia coli, the major causative agent of urinary tract infections in humans. Fimbriae share a common mode of biogenesis, orchestrated by a molecular assembly platform called ‘the usher’ located in the outer membrane. Although the mechanism of pilus biogenesis is well characterized, how the usher itself is assembled at the outer membrane is unclear. Here, we report that a rapid response in usher assembly is crucially dependent on the translocation assembly module. We assayed the assembly reaction for a range of ushers and provide mechanistic insight into the β-barrel assembly pathway that enables the rapid deployment of bacterial fimbriae.

Type: Article
Title: Effective assembly of fimbriae in Escherichia coli depends on the translocation assembly module nanomachine
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/NMICROBIOL.2016.64
Publisher version: http://dx.doi.org/10.1038/NMICROBIOL.2016.64
Language: English
Additional information: Copyright © 2016 Macmillan Publishers Limited. All rights reserved.
Keywords: Bacteriology, Pathogens
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1514831
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