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Fascin Regulates Nuclear Movement and Deformation in Migrating Cells

Jayo, A; Malboubi, M; Antoku, S; Chang, W; Ortiz-Zapater, E; Groen, C; Pfisterer, K; ... Parsons, M; + view all (2016) Fascin Regulates Nuclear Movement and Deformation in Migrating Cells. Developmental Cell , 38 (4) pp. 371-383. 10.1016/j.devcel.2016.07.021. Green open access

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Abstract

Fascin is an F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating cells, and its expression is correlated with poor prognosis and increased metastatic potential in a number of cancers. Here, we identified the nuclear envelope protein nesprin-2 as a binding partner for fascin in a range of cell types in vitro and in vivo. Nesprin-2 interacts with fascin through a direct, F-actin-independent interaction, and this binding is distinct and separable from a role for fascin within filopodia at the cell periphery. Moreover, disrupting the interaction between fascin and nesprin-2 C-terminal domain leads to specific defects in F-actin coupling to the nuclear envelope, nuclear movement, and the ability of cells to deform their nucleus to invade through confined spaces. Together, our results uncover a role for fascin that operates independently of filopodia assembly to promote efficient cell migration and invasion.

Type: Article
Title: Fascin Regulates Nuclear Movement and Deformation in Migrating Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.devcel.2016.07.021
Publisher version: http://dx.doi.org/10.1016/j.devcel.2016.07.021
Language: English
Additional information: © 2016 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords: Science & Technology, Life Sciences & Biomedicine, Cell Biology, Developmental Biology, TAN LINE FORMATION, ACTIN FLOW, CANCER-CELLS, PROTEIN, FILOPODIA, ENVELOPE, ADHESION, METASTASIS, MECHANISM, INVASION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1513748
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