Jayo, A;
Malboubi, M;
Antoku, S;
Chang, W;
Ortiz-Zapater, E;
Groen, C;
Pfisterer, K;
... Parsons, M; + view all
(2016)
Fascin Regulates Nuclear Movement and Deformation in Migrating Cells.
Developmental Cell
, 38
(4)
pp. 371-383.
10.1016/j.devcel.2016.07.021.
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Abstract
Fascin is an F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating cells, and its expression is correlated with poor prognosis and increased metastatic potential in a number of cancers. Here, we identified the nuclear envelope protein nesprin-2 as a binding partner for fascin in a range of cell types in vitro and in vivo. Nesprin-2 interacts with fascin through a direct, F-actin-independent interaction, and this binding is distinct and separable from a role for fascin within filopodia at the cell periphery. Moreover, disrupting the interaction between fascin and nesprin-2 C-terminal domain leads to specific defects in F-actin coupling to the nuclear envelope, nuclear movement, and the ability of cells to deform their nucleus to invade through confined spaces. Together, our results uncover a role for fascin that operates independently of filopodia assembly to promote efficient cell migration and invasion.
| Type: | Article |
|---|---|
| Title: | Fascin Regulates Nuclear Movement and Deformation in Migrating Cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.devcel.2016.07.021 |
| Publisher version: | http://dx.doi.org/10.1016/j.devcel.2016.07.021 |
| Language: | English |
| Additional information: | © 2016 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Cell Biology, Developmental Biology, TAN LINE FORMATION, ACTIN FLOW, CANCER-CELLS, PROTEIN, FILOPODIA, ENVELOPE, ADHESION, METASTASIS, MECHANISM, INVASION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1513748 |
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