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Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A

Fernandez, B; Fdez, E; Gomez-Suaga, P; Gil, F; Molina-Villalba, I; Ferrer, I; Patel, S; ... Hilfiker, S; + view all (2016) Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A. Autophagy , 12 (9) pp. 1487-1506. 10.1080/15548627.2016.1190072. Green open access

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Abstract

Various neurodegenerative disorders are associated with increased brain iron content. Iron is known to cause oxidative stress, which concomitantly promotes cell death. Whereas endolysosomes are known to serve as intracellular iron storage organelles, the consequences of increased iron on endolysosomal functioning, and effects on cell viability upon modulation of endolysosomal iron release remain largely unknown. Here, we show that increasing intracellular iron causes endolysosomal alterations associated with impaired autophagic clearance of intracellular protein aggregates, increased cytosolic oxidative stress and increased cell death. These effects are subject to regulation by NAADP, a potent second messenger reported to target endolysosomal TPCNs (2-pore channels). Consistent with endolysosomal iron storage, cytosolic iron levels are modulated by NAADP, and increased cytosolic iron is detected when overexpressing active, but not inactive TPCNs, indicating that these channels can modulate endolysosomal iron release. Cell death triggered by altered intralysosomal iron handling is abrogated in the presence of an NAADP antagonist or when inhibiting RAB7A activity. Taken together, our results suggest that increased endolysosomal iron causes cell death associated with increased cytosolic oxidative stress as well as autophagic impairments, and these effects are subject to modulation by endolysosomal ion channel activity in a RAB7A-dependent manner. These data highlight alternative therapeutic strategies for neurodegenerative disorders associated with increased intracellular iron load.

Type: Article
Title: Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/15548627.2016.1190072
Publisher version: http://doi.org/10.1080/15548627.2016.1190072
Language: English
Additional information: Copyright © 2016 The Author(s). Published withlicense by Taylor & Francis Group, LLC©Belén Fernández, Elena Fdez, PatriciaGómez-Suaga, Fernando Gil, Isabel Molina-Villalba, Isidro Ferrer, Sandip Patel, Grant C.Churchill, and Sabine Hilfiker & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
Keywords: iron, lysosome, NAADP, neurodegeneration, RAB7A, TPCN1, TPCN2
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1509534
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