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Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells

Brault, A; Rallis, C; Normant, V; Garant, JM; Bähler, J; Labbé, S; (2016) Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells. G3 , 6 (10) pp. 3077-3095. 10.1534/g3.116.031898. Green open access

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Abstract

Meiosis is essential for sexually reproducing organisms, including the fission yeast Schizosaccharomyces pombe In meiosis, chromosomes replicate once in diploid precursor cell (zygote), and then segregate twice to generate four haploid meiotic products, named spores in yeast. In S. pombe, Php4 is responsible for the transcriptional repression capability of the heteromeric CCAAT-binding factor to negatively regulate genes encoding iron-using proteins under low-iron conditions. Here, we show that the CCAAT-regulatory subunit Php4 is required for normal progression of meiosis under iron-limiting conditions. Cells lacking Php4 exhibit a meiotic arrest at metaphase I. Microscopic analyses of cells expressing functional GFP-Php4 show that it co-localizes with chromosomal material at every stage of meiosis under low concentrations of iron. In contrast, GFP-Php4 fluorescence signal is lost when cells undergo meiosis under iron-replete conditions. Global gene expression analysis of meiotic cells using DNA microarrays identified 137 genes that are regulated in an iron- and Php4-dependent manner. Among them, 18 genes are expressed exclusively during meiosis and constitute new putative Php4 target genes, which include hry1(+) and mug14(+) Further analysis validates that Php4 is required for maximal and timely repression of hry1(+) and mug14(+) genes. Using a chromatin immunoprecipitation approach, we show that Php4 specifically associates with hry1(+) and mug14(+) promoters in vivo Taken together, the results reveal that in iron-starved meiotic cells, Php4 is essential for completion of the meiotic program since it participates in a global gene expression reprogramming to optimize the use of limited available iron.

Type: Article
Title: Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1534/g3.116.031898
Publisher version: http://dx.doi.org/10.1534/g3.116.031898
Language: English
Additional information: Copyright © 2016 Brault et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: CCAAT-binding factor, fission yeast, iron-regulated genes, iron-sparing response, meiosis
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/1508919
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