UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Electrostatic swelling of bicontinuous cubic lipid phases

Tyler, AII; Barriga, HMG; Parsons, ES; McCarthy, NLC; Ces, O; Law, RV; Seddon, JM; (2015) Electrostatic swelling of bicontinuous cubic lipid phases. Soft Matter , 11 (16) pp. 3279-3286. 10.1039/C5SM00311C. Green open access

[img]
Preview
Text (Article)
Parsons_c5sm00311c.pdf

Download (534kB) | Preview
[img]
Preview
Text (Supplementary Information)
Parsons_c5sm00311c1.pdf

Download (2MB) | Preview

Abstract

Lipid bicontinuous cubic phases have attracted enormous interest as bio-compatible scaffolds for use in a wide range of applications including membrane protein crystallisation, drug delivery and biosensing. One of the major bottlenecks that has hindered exploitation of these structures is an inability to create targeted highly swollen bicontinuous cubic structures with large and tunable pore sizes. In contrast, cubic structures found in vivo have periodicities approaching the micron scale. We have been able to engineer and control highly swollen bicontinuous cubic phases of spacegroup Im3m containing only lipids by (a) increasing the bilayer stiffness by adding cholesterol and (b) inducing electrostatic repulsion across the water channels by addition of anionic lipids to monoolein. By controlling the composition of the ternary mixtures we have been able to achieve lattice parameters up to 470 Å, which is 5 times that observed in pure monoolein and nearly twice the size of any lipidic cubic phase reported previously. These lattice parameters significantly exceed the predicted maximum swelling for bicontinuous cubic lipid structures, which suggest that thermal fluctuations should destroy such phases for lattice parameters larger than 300 Å.

Type: Article
Title: Electrostatic swelling of bicontinuous cubic lipid phases
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/C5SM00311C
Publisher version: http://dx.doi.org/10.1039/C5SM00311C
Language: English
Additional information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1508327
Downloads since deposit
104Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item