UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Phospho-dependent Accumulation of GABABRs at Presynaptic Terminals after NMDAR Activation

Hannan, S; Gerrow, K; Triller, A; Smart, TG; (2016) Phospho-dependent Accumulation of GABABRs at Presynaptic Terminals after NMDAR Activation. Cell Reports , 16 (7) pp. 1962-1973. 10.1016/j.celrep.2016.07.021. Green open access

[img]
Preview
Text
smart_1-s2.0-S2211124716309202-main.pdf

Download (3MB) | Preview

Abstract

Here, we uncover a mechanism for regulating the number of active presynaptic GABAB receptors (GABABRs) at nerve terminals, an important determinant of neurotransmitter release. We find that GABABRs gain access to axon terminals by lateral diffusion in the membrane. Their relative accumulation is dependent upon agonist activation and the presence of the two distinct sushi domains that are found only in alternatively spliced GABABR1a subunits. Following brief activation of NMDA receptors (NMDARs) using glutamate, GABABR diffusion is reduced, causing accumulation at presynaptic terminals in a Ca2+-dependent manner that involves phosphorylation of GABABR2 subunits at Ser783. This signaling cascade indicates how synaptically released glutamate can initiate, via a feedback mechanism, increased levels of presynaptic GABABRs that limit further glutamate release and excitotoxicity.

Type: Article
Title: Phospho-dependent Accumulation of GABABRs at Presynaptic Terminals after NMDAR Activation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.celrep.2016.07.021
Publisher version: http://doi.org/10.1016/j.celrep.2016.07.021
Language: English
Additional information: © 2016 The Author(s). This is an open access article under the Creative Commons Attribution 4.0 International (CC BY 4.0) license (http://creativecommons.org/licenses/by/4.0/)
Keywords: Science & Technology, Life Sciences & Biomedicine, Cell Biology, GABA(B) RECEPTOR INTERNALIZATION, GAMMA-AMINOBUTYRIC-ACID, CENTRAL-NERVOUS-SYSTEM, PROTEIN-KINASE, LATERAL MOBILITY, INHIBITORY NEUROTRANSMISSION, HETEROSYNAPTIC DEPRESSION, SURFACE TRAFFICKING, SYNAPTIC PLASTICITY, SUSHI DOMAINS; AMPK; bungarotoxin binding site; Ca2+ signaling; excitotoxicity; GABA receptors; GABAB receptors; glutamate receptors; hippocampus; homeostatic signaling; lateral diffusion; NMDA receptors; phosphorylation; presynaptic terminal; quantum dots; receptor mobility; single-particle tracking; sushi domains
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1508144
Downloads since deposit
34Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item