UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Divergent axon collaterals in the regenerating goldfish optic tract: a fluorescence double-label study.

Becker, DL; Cook, JE; (1988) Divergent axon collaterals in the regenerating goldfish optic tract: a fluorescence double-label study. Development , 104 (2) pp. 317-320.

Full text not available from this repository.

Abstract

In the normal goldfish, optic axons are distributed between the two arms (brachia) of each optic tract, in such a way that each axon enters the tectum close to its retinotopic termination site. We have shown previously that regenerating axons at first express little or no preference for their normal brachium. Later, however, a partial refinement of the brachial pathway takes place, implying that some axons must have sent out divergent collateral branches and then eliminated the least appropriate. We have now studied the formation and subsequent loss of axon collaterals in regeneration using retrogradely transported fluorescent dyes. We labelled the axons in the medial brachium with Fast Blue and those in the lateral brachium with Diamidino Yellow in a way that avoided cross-contamination. In normal fish, yellow-labelled ganglion cells dominated the dorsal retina and blue-labelled ganglion cells the ventral, with only a narrow zone of overlap. Double-labelled cells were not found. In fish labelled early in regeneration, however, both dyes were spread over the entire retina in single- and double-labelled ganglion cells. As regeneration progressed, each dye again came to dominate its appropriate retinal region; but much less strongly, confirming previous results. At the same time, double-labelled cells became harder to find. From 60 days after nerve cut onwards they were rare, and largely confined to the boundary zone between dorsal and ventral retina.(ABSTRACT TRUNCATED AT 250 WORDS)

Type: Article
Title: Divergent axon collaterals in the regenerating goldfish optic tract: a fluorescence double-label study.
Location: England
Keywords: Animals, Axons, Collateral Circulation, Cyprinidae, Ganglia, Goldfish, Microscopy, Fluorescence, Nerve Regeneration, Optic Nerve, Retina
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/150589
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item