Storm, MP; Sorrell, I; Shipley, R; Regan, S; Luetchford, KA; Sathish, J; Webb, S; Storm, MP; Sorrell, I; Shipley, R; Regan, S; Luetchford, KA; Sathish, J; Webb, S; Ellis, MJ; - view fewer (2016) Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture. Journal of Visualized Experiments (111) , Article e53431. 10.3791/53431.

Preview

Text jove-111-53431.pdf - Published Version Download (1MB) | Preview

Abstract

Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the functionality of cells grown in 3-dimensional culture systems the cells are shown to possess increased albumin production (an important hepatic function) when compared to standard 2-dimensional tissue culture.

Download activity - last month

Export as JSONCSVXML

Download activity - last 12 months

Export as JSONCSVXML

Downloads by country - last 12 months

Export as XMLCSVJSON

Archive Staff Only

View Item