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Transformation studies on human fibroblasts from familial polyposis coli patients and normal donors.

Delhanty, JD; Rider, SH; (1988) Transformation studies on human fibroblasts from familial polyposis coli patients and normal donors. Mutat Res , 199 (2) pp. 327-339.

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Abstract

Transformation experiments have been carried out on human diploid fibroblasts derived from normal individuals and those from 2 groups with dominantly inherited cancer predisposition, familial polyposis coli (FPC), and multiple endocrine neoplasia, type 2 (MEN-2). Treatment with a single or multiple doses of the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), resulted in limited anchorage-independent (AI) growth in both normal and FPC cultures; no permanent cell lines were produced but FPC cells showed increased proliferation with low doses of the carcinogen. Carcinogen treatment followed by application of the tumour promoter, 12-O-tetradecanoyl phorbol-13-acetate (TPA), for 38 weeks was insufficient to cause full transformation in cultures derived from normal people or MEN-2 patients although AI growth was induced in all 3 cell types. Three FPC cultures exhibited an extended life span over the solvent controls. Two of these are still actively dividing and have a clonal pseudodiploid karyotype.

Type: Article
Title: Transformation studies on human fibroblasts from familial polyposis coli patients and normal donors.
Location: Netherlands
Keywords: Adenomatous Polyposis Coli, Cell Division, Cell Transformation, Neoplastic, Fibroblasts, Humans, Kinetics, Methylnitronitrosoguanidine, Multiple Endocrine Neoplasia, Neoplasms, Skin, Tetradecanoylphorbol Acetate
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health > Reproductive Health
URI: http://discovery.ucl.ac.uk/id/eprint/1501716
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