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CoA synthase influences adherence-independent growth and survival of mammalian cells in vitro

Breus, OS; Nemazanyy, IO; Gout, IT; Filonenko, VV; Panasyuk, GG; (2009) CoA synthase influences adherence-independent growth and survival of mammalian cells in vitro. Biopolymers and Cell , 25 (5) pp. 384-389. 10.7124/bc.0007F0.

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Abstract

© Institute of Molecular Biology and Genetics NAS of Ukraine, 2009. Aims. We aimed to study the influence of the expression level, activity and subcellular localization of CoA Synthase (CoASy) on anchoring independent growth and viability of in vitro cultured cells. Methods. Abilities of cells to form colonies in semisolid agarose and survive in growth factor depleted conditions were tested. A panel of HEK293 stable cell lines which over express wild type, catalytically inactive or mitochondria association mutants of CoASy were used in the study. Effects of CoASy down regulation by siRNA on malignant phenotype of HepG2 cells have been studied. Results. We report here that changes in CoASy expression level affect anchoring independent growth and viability of mammalian cells. Catalytic activity of CoASy and its association with mitochondria are crucial for mediating of the observed effects. Conclusions. Presented data indicate CoASy has positive impact on activity of signalling pathways in the cell and reveal unknown before functional link between signal transduction and metabolism.

Type: Article
Title: CoA synthase influences adherence-independent growth and survival of mammalian cells in vitro
DOI: 10.7124/bc.0007F0
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1501701
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