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Optimization of dietary restriction protocols in Drosophila

Bass, TM; Grandison, RC; Wong, R; Martinez, P; Partridge, L; Piper, MDW; (2007) Optimization of dietary restriction protocols in Drosophila. Journal of Gerontology: Biological Sciences , 62 (10) 1071 - 1081. Green open access

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Abstract

Dietary restriction (DR) extends life span in many organisms, through unknown mechanisms that may or may not be evolutionarily conserved. Because different laboratories use different diets and techniques for implementing DR, the outcomes may not be strictly comparable. This complicates intra- and interspecific comparisons of the mechanisms of DR and is therefore central to the use of model organisms to research this topic. Drosophila melanogaster is an important model for the study of DR, but the nutritional content of its diet is typically poorly defined. We have compared fly diets composed of different yeasts for their effect on life span and fecundity. We found that only one diet was appropriate for DR experiments, indicating that much of the published work on fly ‘‘DR’’ may have included adverse effects of food composition. We propose procedures to ensure that diets are suitable for the study of DR in Drosophila.

Type: Article
Title: Optimization of dietary restriction protocols in Drosophila
Open access status: An open access version is available from UCL Discovery
Publisher version: http://biomedgerontology.oxfordjournals.org/conten...
Language: English
Additional information: This is a pre-copyedited, author-produced PDF of an article accepted for publication in The Journals of Gerontology: Series A following peer review. The version of record for Bass, TM; Grandison, RC; Wong, R; Martinez, P; Partridge, L; Piper, MDW; (2007) Optimization of dietary restriction protocols in Drosophila. Journal of Gerontology: Biological Sciences , 62 (10) 1071 - 1081 is available online at: http://biomedgerontology.oxfordjournals.org/content/62/10/1071.full.pdf+html.
Keywords: Extends life-span, caenorhabditis-elegans, calorie restriction, stress resistance, melanogaster, rats, longevity, female, modulation, ingestion
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/150060
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