Sirianni, A;
Krokowski, S;
Lobato-Márquez, D;
Buranyi, S;
Pfanzelter, J;
Galea, D;
Willis, A;
... Mostowy, S; + view all
(2016)
Mitochondria mediate septin cage assembly to promote autophagy of Shigella.
EMBO Reports
, 17
(7)
pp. 1029-1043.
10.15252/embr.201541832.
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Siranni et al 2016 Mitochondria mediate septin cage assembly to promote autophagy of Shigella VOR.pdf Download (3MB) | Preview |
Abstract
Septins, cytoskeletal proteins with well-characterised roles in cytokinesis, form cage-like structures around cytosolic Shigella flexneri and promote their targeting to autophagosomes. However, the processes underlying septin cage assembly, and whether they influence S. flexneri proliferation, remain to be established. Using single-cell analysis, we show that the septin cages inhibit S. flexneri proliferation. To study mechanisms of septin cage assembly, we used proteomics and found mitochondrial proteins associate with septins in S. flexneri-infected cells. Strikingly, mitochondria associated with S. flexneri promote septin assembly into cages that entrap bacteria for autophagy. We demonstrate that the cytosolic GTPase dynamin-related protein 1 (Drp1) interacts with septins to enhance mitochondrial fission. To avoid autophagy, actin-polymerising Shigella fragment mitochondria to escape from septin caging. Our results demonstrate a role for mitochondria in anti-Shigella autophagy and uncover a fundamental link between septin assembly and mitochondria.
| Type: | Article |
|---|---|
| Title: | Mitochondria mediate septin cage assembly to promote autophagy of Shigella |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.15252/embr.201541832 |
| Publisher version: | http://dx.doi.org/10.15252/embr.201541832 |
| Language: | English |
| Additional information: | Copyright © 2016 The Authors. Published under the terms of the Creative Commons Attribution 4.0 license (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | autophagy; cytoskeleton; mitochondria; septin; Shigella |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1498887 |
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