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A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue

Heywood, WE; Pryce, J; Virasami, A; Preece, RL; Dezateux, C; Mills, K; Sebire, NJ; (2016) A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue. Placenta , 44 pp. 109-111. 10.1016/j.placenta.2016.04.014. Green open access

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Abstract

A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.

Type: Article
Title: A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.placenta.2016.04.014
Publisher version: http://dx.doi.org/10.1016/j.placenta.2016.04.014
Language: English
Additional information: Copyright © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International licence (CC BY-NC-ND 4.0) (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords: Free peptide, LC-MS, Multivariate analysis, Placenta, Proteolysis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Population, Policy and Practice Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1494669
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