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Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells

Szoboszlay, M; Lorincz, A; Lanore, F; Vervaeke, K; Silver, RA; Nusser, Z; (2016) Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells. Neuron , 90 (5) pp. 1043-1056. 10.1016/j.neuron.2016.03.029. Green open access

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Abstract

The strength and variability of electrical synaptic connections between GABAergic interneurons are key determinants of spike synchrony within neuronal networks. However, little is known about how electrical coupling strength is determined due to the inaccessibility of gap junctions on the dendritic tree. We investigated the properties of gap junctions in cerebellar interneurons by combining paired somato-somatic and somato-dendritic recordings, anatomical reconstructions, immunohistochemistry, electron microscopy, and modeling. By fitting detailed compartmental models of Golgi cells to their somato-dendritic voltage responses, we determined their passive electrical properties and the mean gap junction conductance (0.9 nS). Connexin36 immunofluorescence and freeze-fracture replica immunogold labeling revealed a large variability in gap junction size and that only 18% of the 340 channels are open in each plaque. Our results establish that the number of gap junctions per connection is the main determinant of both the strength and variability in electrical coupling between Golgi cells.

Type: Article
Title: Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.neuron.2016.03.029
Publisher version: http://dx.doi.org/10.1016/j.neuron.2016.03.029
Language: English
Additional information: © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Science & Technology, Life Sciences & Biomedicine, Neurosciences, Neurosciences & Neurology, COUPLED INTERNEURON NETWORK, PYRAMIDAL NEURON DENDRITES, PH-DEPENDENT MODULATION, GOLDFISH MAUTHNER CELL, PATCH-CLAMP RECORDINGS, LONG-TERM MODULATION, ELECTRICAL SYNAPSES, GABAERGIC INTERNEURONS, SYNAPTIC INTEGRATION, INHIBITORY INTERNEURONS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1494139
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