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Archaeal TFE alpha/beta is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39

Blombach, F; Salvadori, E; Fouqueau, T; Yan, J; Reimann, J; Sheppard, C; Smollett, KL; ... Werner, F; + view all (2015) Archaeal TFE alpha/beta is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39. ELIFE , 4 (ARTN e08) 10.7554/eLife.08378. Green open access

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Abstract

Transcription initiation of archaeal RNA polymerase (RNAP) and eukaryotic RNAPII is assisted by conserved basal transcription factors. The eukaryotic transcription factor TFIIE consists of α and β subunits. Here we have identified and characterised the function of the TFIIEβ homologue in archaea that on the primary sequence level is related to the RNAPIII subunit hRPC39. Both archaeal TFEβ and hRPC39 harbour a cubane 4Fe-4S cluster, which is crucial for heterodimerization of TFEα/β and its engagement with the RNAP clamp. TFEα/β stabilises the preinitiation complex, enhances DNA melting, and stimulates abortive and productive transcription. These activities are strictly dependent on the β subunit and the promoter sequence. Our results suggest that archaeal TFEα/β is likely to represent the evolutionary ancestor of TFIIE-like factors in extant eukaryotes.

Type: Article
Title: Archaeal TFE alpha/beta is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.08378
Publisher version: http://dx.doi.org/ 10.7554/eLife.08378
Language: English
Additional information: © Copyright Blombach et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Keywords: Transcription Factor-iie, Open Complex-formation, Ray Crystal-structure, Turn-helix Domain, Hyperthermophilic Archaeon, Sulfolobus-solfataricus, Promoter Recognition, Gene-regulation, Initiation, Dna
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1492447
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