Hershkovitz, I; Donoghue, HD; Minnikin, DE; Besra, GS; Lee, OYC; Gernaey, AM; ... Spigelman, M; + view all Hershkovitz, I; Donoghue, HD; Minnikin, DE; Besra, GS; Lee, OYC; Gernaey, AM; Galili, E; Eshed, V; Greenblatt, CL; Lemma, E; Bar-Gal, GK; Spigelman, M; - view fewer (2008) Detection and Molecular Characterization of 9000-Year-Old Mycobacterium tuberculosis from a Neolithic Settlement in the Eastern Mediterranean. PLOS ONE , 3 (10) , Article e3426. 10.1371/journal.pone.0003426.
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Background: Mycobacterium tuberculosis is the principal etiologic agent of human tuberculosis. It has no environmental reservoir and is believed to have co-evolved with its host over millennia. This is supported by skeletal evidence of the disease in early humans, and inferred from M. tuberculosis genomic analysis. Direct examination of ancient human remains for M. tuberculosis biomarkers should aid our understanding of the nature of prehistoric tuberculosis and the host/pathogen relationship.Methodology/Principal Findings: We used conventional PCR to examine bone samples with typical tuberculosis lesions from a woman and infant, who were buried together in the now submerged site of Atlit-Yam in the Eastern Mediterranean, dating from 9250-8160 years ago. Rigorous precautions were taken to prevent contamination, and independent centers were used to confirm authenticity of findings. DNA from five M. tuberculosis genetic loci was detected and had characteristics consistent with extant genetic lineages. High performance liquid chromatography was used as an independent method of verification and it directly detected mycolic acid lipid biomarkers, specific for the M. tuberculosis complex.Conclusions/Significance: Human tuberculosis was confirmed by morphological and molecular methods in a population living in one of the first villages with evidence of agriculture and animal domestication. The widespread use of animals was not a source of infection but may have supported a denser human population that facilitated transmission of the tubercle bacillus. The similarity of the M. tuberculosis genetic signature with those of today gives support to the theory of a long-term co-existence of host and pathogen.
|Title:||Detection and Molecular Characterization of 9000-Year-Old Mycobacterium tuberculosis from a Neolithic Settlement in the Eastern Mediterranean|
|Open access status:||An open access version is available from UCL Discovery|
|Additional information:||© 2008 Hershkovitz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Keywords:||POLYMERASE-CHAIN-REACTION, PEDIATRIC TUBERCULOSIS, INFECTIOUS-DISEASES, SKELETAL REMAINS, ANCIENT DNA, COMPLEX DNA, EPIDEMIOLOGY, POPULATIONS, OSTEOARTHROPATHY, PHYLOGENY|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)|
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