Tian, F and Gourine, AV and Huckstepp, RT and Dale, N (2009) A microelectrode biosensor for real time monitoring of L-glutamate release. Anal Chim Acta , 645 (1-2) 86 - 91. 10.1016/j.aca.2009.04.048.
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We have developed an amperometric microbiosensor for real time monitoring L-glutamate release in neural tissue, based on enzymatic oxidation catalyzed by the L-glutamate oxidase. By means of a sol-gel coating method, L-glutamate oxidase was entrapped in a biocompatible gel layer that provided a benign environment and retained enzyme activity on the surface of Pt microelectrode. Prior to gel layer formation, a modification on the surface of Pt microelectrode with poly(phenylene diamine) enabled the microbiosensor screen majority of common potential interfering substances existing in physiological samples. The miniaturized biosensor achieved a steady state response to l-glutamate within 10 s and exhibited a linear dependence on the concentration of L-glutamate from 0.5 to 100 micromol L(-1) with a high sensitivity of 279.4 +/- 2.0 microA (mmol L(-1))(-1) cm(-2) (n = 4, R.S.D. = 2.8%). The microbiosensor also exhibited excellent long-term stability in dry storage. We have successfully used the microbiosensor for real time measuring of L-glutamate in vivo.
|Title:||A microelectrode biosensor for real time monitoring of L-glutamate release.|
|Keywords:||Amino Acid Oxidoreductases, Animals, Biosensing Techniques, Enzymes, Immobilized, Glutamic Acid, Male, Microelectrodes, Phase Transition, Rats, Rats, Sprague-Dawley, Sensitivity and Specificity, Solitary Nucleus, Streptomyces|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Neuroscience, Physiology and Pharmacology|
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