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Imperfection and radiation damage in protein crystals studied with coherent radiation

Nave, C; Sutton, G; Evans, G; Owen, R; Rau, C; Robinson, I; Stuart, DI; (2016) Imperfection and radiation damage in protein crystals studied with coherent radiation. Journal of Synchrotron Radiation , 23 (1) pp. 228-237. 10.1107/S1600577515019700. Green open access

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Abstract

Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub-micrometre scale. In addition, these features can provide more accurate intensity measurements (e.g. by model-based profile fitting), detwinning (by distinguishing the various components), phasing (by exploiting sampling of the molecular transform) and refinement (by distinguishing regions with different unit-cell parameters). In order to exploit these potential benefits, the features due to coherent diffraction have to be recorded and any change due to radiation damage properly modelled. Initial results from recording coherent diffraction at cryotemperatures from polyhedrin crystals of approximately 2 µm in size are described. These measurements allowed information about the type of crystal imperfections to be obtained at the sub-micrometre level, together with the changes due to radiation damage.

Type: Article
Title: Imperfection and radiation damage in protein crystals studied with coherent radiation
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1107/S1600577515019700
Publisher version: http://dx.doi.org/10.1107/S1600577515019700
Language: English
Additional information: Copyright © The Authors 2016. This is an Open Access article published under the Creative Commons Attribution 2.0 UK: England & Wales Licence (https://creativecommons.org/licenses/by/2.0/uk/).
Keywords: coherent diffraction, crystal perfection, radiation damage
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1487063
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