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Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation.

Salim, K; Fenton, T; Bacha, J; Urien-Rodriguez, H; Bonnert, T; Skynner, HA; Watts, E; ... Guest, PC; + view all (2002) Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation. J Biol Chem , 277 (18) pp. 15482-15485. 10.1074/jbc.M201539200.

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Abstract

Recent studies have shown that G-protein-coupled receptors (GPCRs) can assemble as high molecular weight homo- and hetero-oligomeric complexes. This can result in altered receptor-ligand binding, signaling, or intracellular trafficking. We have co-transfected HEK-293 cells with differentially epitope-tagged GPCRs from different subfamilies and determined whether oligomeric complexes were formed by co-immunoprecipitation and immunoblot analysis. This gave the surprising result that the 5HT(1A) receptor was capable of forming hetero-oligomers with all GPCRs tested including the 5HT(1B), 5HT(1D), EDG(1), EDG(3), GPR(26), and GABA(B2) receptors. The testing of other GPCR combinations showed similar results with hetero-oligomer formation occurring for the 5HT(1D) with the 5HT(1B) and EDG(1) receptor. Control studies showed that these complexes were present in co-transfected cells before the time of lysis and that the hetero-oligomers were comprised of GPCRs at discrete stoichiometries. These findings suggest that GPCRs have a natural tendency to form oligomers when co-transfected into cells. Future studies should therefore investigate the presence and physiological role of GPCR hetero-oligomers in cells in which they are endogenously expressed.

Type: Article
Title: Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation.
Location: United States
DOI: 10.1074/jbc.M201539200
Keywords: Cell Line, GTP-Binding Proteins, Humans, Immunoblotting, Kidney, Mutagenesis, Site-Directed, Protein Subunits, Proto-Oncogene Proteins c-myc, Receptor, Serotonin, 5-HT1B, Receptor, Serotonin, 5-HT1D, Receptors, Cell Surface, Receptors, Serotonin, Receptors, Serotonin, 5-HT1, Recombinant Proteins, Restriction Mapping, Transfection
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: http://discovery.ucl.ac.uk/id/eprint/1484214
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