UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides.

Buckingham, SD; Pym, L; Sattelle, DB; (2006) Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides. Methods Mol Biol , 322 pp. 331-345. 10.1007/978-1-59745-000-3_23.

Full text not available from this repository.

Abstract

The Xenopus laevis oocyte offers one of the most convenient expression systems for assaying the actions of candidate ligands on cloned ionotropic neurotransmitter receptors (also known as ligand-gated ion channels [LGICs]). Their large size makes injection of complementary ribonucleic acid or complementary deoxyribonucleic acid and electrophysiological recording very easy. Furthermore, Xenopus oocytes translate messages very efficiently, resulting in the detection of large-amplitude ligand-induced currents from expressed, recombinant LGICs. Compared to other electrophysiological techniques, recording from oocytes is not difficult and requires only a basic electrophysiological recording setup. Oocytes can be used for two-electrode voltage clamp, as well as cell-attached patch and inside- or outside-out patch clamp recordings. A variety of protocols allows the experimenter to determine the actions of ligands on cloned receptors and parameters, such as their affinity, efficacy, rates of association and desensitization, and reversibility, to be estimated. Here, we present protocols for using Xenopus oocytes in assaying candidate ligands acting against cloned targets of drugs and pesticides.

Type: Article
Title: Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides.
Location: United States
DOI: 10.1007/978-1-59745-000-3_23
Keywords: Animals, Female, Insecticides, Ion Channel Gating, Ligands, Oocytes, Patch-Clamp Techniques, Receptors, Neurotransmitter, Xenopus laevis
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/1482935
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item