UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Identification of adult nephron progenitors capable of kidney regeneration in zebrafish.

Diep, CQ; Ma, D; Deo, RC; Holm, TM; Naylor, RW; Arora, N; Wingert, RA; ... Davidson, AJ; + view all (2011) Identification of adult nephron progenitors capable of kidney regeneration in zebrafish. Nature , 470 (7332) pp. 95-100. 10.1038/nature09669.

Full text not available from this repository.

Abstract

Loss of kidney function underlies many renal diseases. Mammals can partly repair their nephrons (the functional units of the kidney), but cannot form new ones. By contrast, fish add nephrons throughout their lifespan and regenerate nephrons de novo after injury, providing a model for understanding how mammalian renal regeneration may be therapeutically activated. Here we trace the source of new nephrons in the adult zebrafish to small cellular aggregates containing nephron progenitors. Transplantation of single aggregates comprising 10-30 cells is sufficient to engraft adults and generate multiple nephrons. Serial transplantation experiments to test self-renewal revealed that nephron progenitors are long-lived and possess significant replicative potential, consistent with stem-cell activity. Transplantation of mixed nephron progenitors tagged with either green or red fluorescent proteins yielded some mosaic nephrons, indicating that multiple nephron progenitors contribute to a single nephron. Consistent with this, live imaging of nephron formation in transparent larvae showed that nephrogenic aggregates form by the coalescence of multiple cells and then differentiate into nephrons. Taken together, these data demonstrate that the zebrafish kidney probably contains self-renewing nephron stem/progenitor cells. The identification of these cells paves the way to isolating or engineering the equivalent cells in mammals and developing novel renal regenerative therapies.

Type: Article
Title: Identification of adult nephron progenitors capable of kidney regeneration in zebrafish.
Location: England
DOI: 10.1038/nature09669
Keywords: Aging, Animals, Animals, Genetically Modified, Cell Proliferation, Kidney, Larva, Models, Animal, Nephrons, Organogenesis, Regeneration, Stem Cell Transplantation, Stem Cells, Zebrafish
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1482061
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item