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Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia

Blanco-Andujar, C; Ortega, D; Southern, P; Nesbitt, SA; Thanh, NT; Pankhurst, QA; (2016) Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia. Nanomedicine , 11 (2) pp. 121-136. 10.2217/nnm.15.185. Green open access

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Abstract

AIM: To assess cell death pathways in response to magnetic hyperthermia. MATERIALS & METHODS: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. RESULTS: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. CONCLUSION: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.

Type: Article
Title: Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.2217/nnm.15.185
Publisher version: http://dx.doi.org/10.2217/nnm.15.185
Additional information: © Nguyễn Thị Kim Thanh. This work is licensed under a Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: apoptosis, cell death pathways, human melanoma cells, magnetic hyperthermia, magnetic nanoparticles
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1479393
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