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Genetic dissection of Down syndrome-associated congenital heart defects using a new mouse mapping panel

Lana-Elola, E; Watson-Scales, S; Slender, A; Gibbins, D; Martineau, A; Douglas, C; Mohun, T; ... Tybulewicz, VLJ; + view all (2016) Genetic dissection of Down syndrome-associated congenital heart defects using a new mouse mapping panel. eLife , 5 , Article e11614. 10.7554/eLife.11614. Green open access

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Abstract

Down syndrome (DS), caused by trisomy of human chromosome 21 (Hsa21), is the most common cause of congenital heart defects (CHD), yet the genetic and mechanistic causes of these defects remain unknown. To identify dosage-sensitive genes that cause DS phenotypes, including CHD, we used chromosome engineering to generate a mapping panel of 7 mouse strains with partial trisomies of regions of mouse chromosome 16 orthologous to Hsa21. Using high-resolution episcopic microscopy and three-dimensional modeling we show that these strains accurately model DS CHD. Systematic analysis of the 7 strains identified a minimal critical region sufficient to cause CHD when present in 3 copies, and showed that it contained at least two dosage-sensitive loci. Furthermore, two of these new strains model a specific subtype of atrio-ventricular septal defects with exclusive ventricular shunting and demonstrate that, contrary to current hypotheses, these CHD are not due to failure in formation of the dorsal mesenchymal protrusion.

Type: Article
Title: Genetic dissection of Down syndrome-associated congenital heart defects using a new mouse mapping panel
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.11614
Publisher version: http://dx.doi.org/10.7554/eLife.11614
Language: English
Additional information: Copyright © Lana-Elola et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Available here: http://creativecommons.org/licenses/by/4.0/
Keywords: Down syndrome, chromosome engineering, congenital heart defects, developmental biology, human biology, medicine, mouse, mouse genetics, stem cells
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Neurology
UCL > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Neurology > Neurodegenerative Diseases
URI: http://discovery.ucl.ac.uk/id/eprint/1478106
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