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A continuum model of transcriptional bursting

Corrigan, AM; Tunnacliffe, E; Cannon, D; Chubb, JR; (2016) A continuum model of transcriptional bursting. eLife , 5 , Article e13051. 10.7554/eLife.13051. Green open access

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Abstract

Transcription occurs in stochastic bursts. Early models based upon RNA hybridisation studies suggest bursting dynamics arise from alternating inactive and permissive states. Here we investigate bursting mechanism in live cells by quantitative imaging of actin gene transcription, combined with molecular genetics, stochastic simulation and probabilistic modelling. In contrast to early models, our data indicate a continuum of transcriptional states, with a slowly fluctuating initiation rate converting the gene between different levels of activity, interspersed with extended periods of inactivity. We place an upper limit of 40s on the lifetime of fluctuations in elongation rate, with initiation rate variations persisting an order of magnitude longer. TATA mutations reduce the accessibility of high activity states, leaving the lifetime of on- and off-states unchanged. A continuum or spectrum of gene states potentially enables a wide dynamic range for cell responses to stimuli.

Type: Article
Title: A continuum model of transcriptional bursting
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.13051
Publisher version: http://dx.doi.org/10.1017/jsc.2014.6
Language: English
Additional information: Copyright © Corrigan et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Keywords: Chromosomes, computational biology, dictyostelium, genes, systems biology
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1477536
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