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Towards single particle imaging of human chromosomes at SACLA

Robinson, I; Schwenke, J; Yusuf, M; Estandarte, A; Zhang, F; Chen, B; Clark, J; ... Fukui, K; + view all (2015) Towards single particle imaging of human chromosomes at SACLA. Journal of Physics B: Atomic, Molecular and Optical Physics , 48 (24) , Article 244007. 10.1088/0953-4075/48/24/244007. Green open access

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Abstract

Single particle imaging (SPI) is one of the front-page opportunities which were used to motivate the construction of the first x-ray free electron lasers (XFELs). SPI's big advantage is that it avoids radiation damage to biological samples because the diffraction takes place in femtosecond single shots before any atomic motion can take place in the sample, hence before the onset of radiation damage. This is the 'diffract before destruction' theme, destruction being assured from the high x-ray doses used. This article reports our collaboration's first attempt at SPI using the SACLA XFEL facility in June 2015. The report is limited to experience with the instrumentation and examples of data because we have not yet had time to invert them to images.

Type: Article
Title: Towards single particle imaging of human chromosomes at SACLA
Open access status: An open access version is available from UCL Discovery
DOI: 10.1088/0953-4075/48/24/244007
Publisher version: http://dx.doi.org/10.1088/0953-4075/48/24/244007
Additional information: © 2015 IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
Keywords: chromosome, radiation damage, diffraction, metaphase, iterative phasing, coherence, imaging
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1477476
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