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Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts

Ibrahim, IM; Puthiyaveetil, S; Khan, C; Allen, JF; (2016) Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts. Photosynthesis Research , 130 (1-3) pp. 93-101. 10.1007/s11120-016-0229-y. Green open access

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Abstract

Two-component signal transduction systems mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Each two-component system consists of a sensor histidine kinase and a response regu- lator. Chloroplast sensor kinase (CSK) is a modified sensor histidine kinase found in chloroplasts—photosynthetic organelles of plants and algae. CSK regulates the tran- scription of chloroplast genes in response to changes in photosynthetic electron transport. In this study, the full- length and truncated forms of Arabidopsis CSK proteins were overexpressed and purified in order to characterise their kinase and redox sensing activities. Our results show that CSK contains a modified kinase catalytic domain that binds ATP with high affinity and forms a quinone adduct that may confer redox sensing activity.

Type: Article
Title: Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s11120-016-0229-y
Publisher version: http://dx.doi.org/ 10.1007/s11120-016-0229-y
Language: English
Additional information: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Keywords: Two-component regulatory system, Sensor histidine kinase, Response regulator, Chloroplast sensor kinase, Plastoquinone, Redox regulation, Chloroplast DNA, Transcription, Cyanobacteria, Photosystem stoichiometry
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/1474740
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