UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

RdgBα reciprocally transfers PA and PI at ER-PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors

Cockcroft, S; Garner, K; Yadav, S; Gomez-Espinoza, E; Raghu, P; (2016) RdgBα reciprocally transfers PA and PI at ER-PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors. Biochemical Society Transactions , 44 (1) pp. 286-292. 10.1042/BST20150228. Green open access

[thumbnail of biochem Soc trans 2015 Review v13 with Fig.pdf]
Preview
Text
biochem Soc trans 2015 Review v13 with Fig.pdf

Download (2MB) | Preview

Abstract

Phosphatidylinositol (PI) is the precursor lipid for the synthesis of PI 4,5-bisphosphate [PI(4,5)P2] at the plasma membrane (PM) and is sequentially phosphorylated by the lipid kinases, PI 4-kinase and phosphatidylinositol 4-phosphate (PI4P)-5-kinase. Receptor-mediated hydrolysis of PI(4,5)P2 takes place at the PM but PI resynthesis occurs at the endoplasmic reticulum (ER). Thus PI(4,5)P2 resynthesis requires the reciprocal transport of two key intermediates, phosphatidic acid (PA) and PI between the ER and the PM. PI transfer proteins (PITPs), defined by the presence of the PITP domain, can facilitate lipid transfer between membranes; the PITP domain comprises a hydrophobic cavity with dual specificity but accommodates a single phospholipid molecule. The class II PITP, retinal degeneration type B (RdgB)α is a multi-domain protein and its PITP domain can bind and transfer PI and PA. In Drosophila photoreceptors, a well-defined G-protein-coupled phospholipase Cβ (PLCβ) signalling pathway, phototransduction defects resulting from loss of RdgBα can be rescued by expression of the PITP domain provided it is competent for both PI and PA transfer. We propose that RdgBα proteins maintain PI(4,5)P2 homoeostasis after PLC activation by facilitating the reciprocal transport of PA and PI at ER-PM membrane contact sites.

Type: Article
Title: RdgBα reciprocally transfers PA and PI at ER-PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1042/BST20150228
Publisher version: http://dx.doi.org/10.1042/BST20150228
Language: English
Additional information: © 2016 Authors; published by Portland Press Limited.
Keywords: 5-bisphosphate [PI(4,5)P2], endoplasmic reticulum–plasma membrane (ER—PM) contact sites, phosphatidic acid, phosphatidic acid (PA) transport proteins, phosphatidylinositol, phosphatidylinositol 4, phosphatidylinositol transfer protein (PITP), phospholipase C, phototransduction, retinal degeneration type B (RdgB)
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/1473392
Downloads since deposit
170Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item