Servant, A;
Jacobs, I;
Bussy, C;
Fabbro, C;
da Ros, T;
Pach, E;
Ballesteros, B;
... Kostarelos, K; + view all
(2016)
Gadolinium-functionalised multi-walled carbon nanotubes as a T1 contrast agent for MRI cell labelling and tracking.
Carbon
, 97
(C)
pp. 126-133.
10.1016/j.carbon.2015.08.051.
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Abstract
The development of efficient contrast agents for cell labelling coupled with powerful medical imaging techniques is of great interest for monitoring cell trafficking with potential clinical applications such as organ repair and regenerative medicine. In this paper, functionalised multi-walled carbon nanotubes (MWNTs) were engineered for cell labelling in T1-weighted MRI applications. These sophisticated constructs were covalently functionalised with the gadolinium (Gd) chelating agent, diethylene triamine pentaacetic acid (DTPA), enabling tight attachment of Gd atoms onto the nanotube surface. The resulting Gd-labelled MWNTs were found to be stable over 2 weeks in water and mouse serum and high payload of Gd atoms could be loaded onto the nanotubes. The r1 relaxivity of the Gd-MWNTs was a 3-fold higher than of the clinically approved T1 contrast agent Magnevist at a magnetic field strength of 7T. The contrast efficiency, expressed as the r1 relaxivity, of the Gd-MWNTs in Human Umbilical Vein Endothelial cells (HUVEC) was investigated at 7T and was found to be around 6.6 mM−1 s−1. There was no reduction of the contrast efficiency after internalisation in HUVECs, which was imparted to the ability of carbon nanotubes to translocate the cell membrane.
| Type: | Article |
|---|---|
| Title: | Gadolinium-functionalised multi-walled carbon nanotubes as a T1 contrast agent for MRI cell labelling and tracking |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.carbon.2015.08.051 |
| Publisher version: | http://dx.doi.org/10.1016/j.carbon.2015.08.051 |
| Language: | English |
| Additional information: | © 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1473084 |
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