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Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis.

Gardiner, C; Ferreira, YJ; Dragovic, RA; Redman, CWG; Sargent, IL; (2013) Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles , 2 10.3402/jev.v2i0.19671.

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Abstract

Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

Type: Article
Title: Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis.
Location: Sweden
DOI: 10.3402/jev.v2i0.19671
Keywords: extracellular vesicles, light scattering, nanoparticle tracking analysis, standardisation
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/1468522
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