REQUIREMENT FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN EPIDERMAL GROWTH-FACTOR SIGNALING.
1188 - 1190.
Stimulation of phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis is a widespread mechanism for receptor-mediated signaling in eukaryotes. Cytosolic phosphatidylinositol transfer protein (PITP) is necessary for guanosine triphosphate (GTP)-dependent hydrolysis of PIP2 by phospholipase C-beta (PLC-beta), but the role of PITP is unclear. Stimulation of phospholipase C-gamma (PLC-gamma) in A431 human epidermoid carcinoma cells treated with epidermal growth factor (EGF) required PITP. Stimulation of PI-4 kinase in cells treated with EGF also required PITP. Coprecipitation studies revealed an EGF-dependent association of PITP with the EGF receptor, with PI-4 kinase, and with PLC-gamma.
|Title:||REQUIREMENT FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN EPIDERMAL GROWTH-FACTOR SIGNALING|
|Keywords:||PHOSPHOLIPASE-C-GAMMA, TYROSINE PHOSPHORYLATION, FACTOR RECEPTOR, EGF RECEPTOR, A431 CELLS, MONOCLONAL-ANTIBODY, PLASMA-MEMBRANES, BINDING-SITE, BOVINE BRAIN, KINASE|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Neuroscience, Physiology and Pharmacology
Archive Staff Only