COCKCROFT, S and THOMAS, GMH and FENSOME, A and GENY, B and CUNNINGHAM, E and GOUT, I and HILES, I and TOTTY, NF and TRUONG, Q and HSUAN, JJ (1994) PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES. SCIENCE , 263 (5146) 523 - 526.
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Abstract
Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on the cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation of phospholipase D in HL60 cells depleted of their cytosol by permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 and ARF3. Recombinant ARF1 substituted for purified ARF proteins in the reconstitution assay. These results indicate that phospholipase D is a downstream effector of ARF1 and ARF3. The well-established role of ARF in vesicular traffic would suggest that alterations in lipid content by PLD are an important determinant in vesicular dynamics.
| Type: | Article |
|---|---|
| Title: | PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES |
| Keywords: | ADP-RIBOSYLATION FACTOR, GTP-BINDING PROTEIN, GOLGI MEMBRANES, NEUTROPHILS, ACTIVATION, VESICLES, FLUORIDE, BOVINE, CELLS, BRAIN |
| UCL classification: | UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Neuroscience, Physiology and Pharmacology |
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