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PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES

COCKCROFT, S; THOMAS, GMH; FENSOME, A; GENY, B; CUNNINGHAM, E; GOUT, I; ... HSUAN, JJ; + view all (1994) PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES. SCIENCE , 263 (5146) 523 - 526.

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Abstract

Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on the cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation of phospholipase D in HL60 cells depleted of their cytosol by permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 and ARF3. Recombinant ARF1 substituted for purified ARF proteins in the reconstitution assay. These results indicate that phospholipase D is a downstream effector of ARF1 and ARF3. The well-established role of ARF in vesicular traffic would suggest that alterations in lipid content by PLD are an important determinant in vesicular dynamics.

Type:Article
Title:PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES
Keywords:ADP-RIBOSYLATION FACTOR, GTP-BINDING PROTEIN, GOLGI MEMBRANES, NEUTROPHILS, ACTIVATION, VESICLES, FLUORIDE, BOVINE, CELLS, BRAIN
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Neuroscience, Physiology and Pharmacology

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