THOMAS, GMH and CUNNINGHAM, E and FENSOME, A and BALL, A and TOTTY, NF and TRUONG, O and HSUAN, JJ and COCKCROFT, S (1993) AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN PHOSPHOLIPASE C-MEDIATED INOSITOL LIPID SIGNALING. CELL , 74 (5) 919 - 928.
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Transmembrane signaling by the phospholipase C-beta (PLC-beta) pathway is known to require at least three components: the receptor, the G protein, and the PLC. Recent studies have indicated that if the cytosol is allowed to leak out of HL60 cells, then G protein-stimulated PLC activity is greatly diminished, indicating an essential role for a cytosolic component(s). We now report the complete purification of one component based on its ability to reconstitute GTPgammaS-mediated PLC activity and identify it as the phosphatidylinositol transfer protein (PI-TP). Based on the in vitro effects of PI-TP, we surmise that it is involved in transporting PI from intracellular compartments for conversion to PI bisphosphate (PIP2) prior to hydrolysis by PLC-beta2/PLC-beta3, the endogenous PLC isoforms present in these cells.
|Title:||AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN PHOSPHOLIPASE C-MEDIATED INOSITOL LIPID SIGNALING|
|Keywords:||BOVINE BRAIN, STREPTOLYSIN-O, ALPHA-SUBUNITS, BETA-GAMMA, CELLS, RECEPTOR, GQ, ACTIVATION, MEMBRANE, BINDING|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology|
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Neuroscience, Physiology and Pharmacology
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