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AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN PHOSPHOLIPASE C-MEDIATED INOSITOL LIPID SIGNALING

THOMAS, GMH; CUNNINGHAM, E; FENSOME, A; BALL, A; TOTTY, NF; TRUONG, O; HSUAN, JJ; (1993) AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN PHOSPHOLIPASE C-MEDIATED INOSITOL LIPID SIGNALING. CELL , 74 (5) 919 - 928.

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Abstract

Transmembrane signaling by the phospholipase C-beta (PLC-beta) pathway is known to require at least three components: the receptor, the G protein, and the PLC. Recent studies have indicated that if the cytosol is allowed to leak out of HL60 cells, then G protein-stimulated PLC activity is greatly diminished, indicating an essential role for a cytosolic component(s). We now report the complete purification of one component based on its ability to reconstitute GTPgammaS-mediated PLC activity and identify it as the phosphatidylinositol transfer protein (PI-TP). Based on the in vitro effects of PI-TP, we surmise that it is involved in transporting PI from intracellular compartments for conversion to PI bisphosphate (PIP2) prior to hydrolysis by PLC-beta2/PLC-beta3, the endogenous PLC isoforms present in these cells.

Type: Article
Title: AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL TRANSFER PROTEIN IN PHOSPHOLIPASE C-MEDIATED INOSITOL LIPID SIGNALING
Keywords: BOVINE BRAIN, STREPTOLYSIN-O, ALPHA-SUBUNITS, BETA-GAMMA, CELLS, RECEPTOR, GQ, ACTIVATION, MEMBRANE, BINDING
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Cell and Developmental Biology
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Neuroscience, Physiology and Pharmacology
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)
URI: http://discovery.ucl.ac.uk/id/eprint/146550
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