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Exploiting the Symmetry of the Resonator Mode to Enhance PELDOR Sensitivity.

Salvadori, E; Fung, MW; Hoffmann, M; Anderson, HL; Kay, CW; (2015) Exploiting the Symmetry of the Resonator Mode to Enhance PELDOR Sensitivity. Appl Magn Reson , 46 (4) 359 - 368. 10.1007/s00723-014-0621-8. Green open access

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Abstract

Pulsed electron paramagnetic resonance (EPR) spectroscopy using microwaves at two frequencies can be employed to measure distances between pairs of paramagnets separated by up to 10 nm. The method, combined with site-directed mutagenesis, has become increasingly popular in structural biology for both its selectivity and capability of providing information not accessible through more standard methods such as nuclear magnetic resonance and X-ray crystallography. Despite these advantages, EPR distance measurements suffer from poor sensitivity. One contributing factor is technical: since 65 MHz typically separates the pump and detection frequencies, they cannot both be located at the center of the pseudo-Lorentzian microwave resonance of a single-mode resonator. To maximize the inversion efficiency, the pump pulse is usually placed at the center of the resonance, while the observer frequency is placed in the wing, with consequent reduction in sensitivity. Here, we consider an alternative configuration: by spacing pump and observer frequencies symmetrically with respect to the microwave resonance and by increasing the quality factor, valuable improvement in the signal-to-noise ratio can be obtained.

Type: Article
Title: Exploiting the Symmetry of the Resonator Mode to Enhance PELDOR Sensitivity.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00723-014-0621-8
Publisher version: http://dx.doi.org/10.1007/s00723-014-0621-8
Language: English
Additional information: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1464979
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