Dawson, DA;
Brekke, P;
Dos Remedios, N;
Horsburgh, GJ;
(2015)
A marker suitable for sex-typing birds from degraded samples.
Conservation Genetics Resources
, 7
(2)
pp. 337-343.
10.1007/s12686-015-0429-3.
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Abstract
A new primer set was developed for sex-typing birds, Z37B. This primer set was designed to amplify alleles of small size to render it suitable for sex-typing degraded samples, including shed feathers. This marker successfully sex-typed 50 % of the species tested, including passerines, shorebirds, rails, seabirds, eagles and the brown kiwi Apteryx australis (allele size range =81–103 bp), and is therefore expected to be suitable for sex-typing a wide range of species. Z37B sex-typed nondegraded samples (blood), degraded tissue (dead unhatched embryos, dead nestlings and museum specimens) and samples of low quantity DNA (plucked feathers and buccal swabs). The small amplicon sizes in birds suggest that this marker will be of utility for sex-typing feathers, swabs and degraded samples from a wide range of avian species.
| Type: | Article |
|---|---|
| Title: | A marker suitable for sex-typing birds from degraded samples |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s12686-015-0429-3 |
| Publisher version: | http://dx.doi.org/10.1007/s12686-015-0429-3 |
| Additional information: | Copyright © The Author(s) 2015. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution,and reproduction in any medium, provided the original author(s) and the source are credited. |
| Keywords: | AVES; Birds; Degraded samples; Feathers; Sex-typing; Non-invasive samples. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1463223 |
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