UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Role of glypican 4 in the regulation of convergent extension movements during gastrulation in Xenopus laevis

Ohkawara, B; Yamamoto, TS; Tada, M; Ueno, N; (2003) Role of glypican 4 in the regulation of convergent extension movements during gastrulation in Xenopus laevis. DEVELOPMENT , 130 (10) 2129 - 2138. 10.1242/dev.00435.

Full text not available from this repository.

Abstract

Coordinated morphogenetic cell movements during gastrulation are crucial for establishing embryonic axes in animals. Most recently, the non-canonical Wnt signaling cascade (PCP pathway) has been shown to regulate convergent extension movements in Xenopus and zebrafish. Heparan sulfate proteoglycans; (HSPGs) are known as modulators of intercellular signaling, and are required for gastrulation movements in vertebrates. However, the function of HSPGs is poorly understood. We analyze the function of Xenopus glypican 4 (Xgly4), which is a member of membrane-associated HSPG family. In situ hybridization revealed that Xgly4 is expressed in the dorsal mesoderm and ectoderm during gastrulation. Reducing the levels of Xgly4 inhibits cell-membrane accumulation of Dishevelled (Dsh), which is a transducer of the Wnt signaling cascade, and thereby disturbs cell movements during gastrulation. Rescue analysis with different Dsh mutants and Wnt11 demonstrated that Xgly4 functions in the non-canonical Wnt/PCP pathway, but not in the canonical Wnt/beta-catenin pathway, to regulate gastrulation movements. We also provide evidence that the Xgly4 protein physically binds Wnt ligands. Therefore, our results suggest that Xgly4 functions as positive regulator in non-canonical Wnt/PCP signaling during gastrulation.

Type: Article
Title: Role of glypican 4 in the regulation of convergent extension movements during gastrulation in Xenopus laevis
DOI: 10.1242/dev.00435
Keywords: heparan sulfate proteoglycan, Wnt signaling pathway, gastrulation movements, Xenopus, HEPARAN-SULFATE PROTEOGLYCANS, PLANAR CELL POLARITY, BONE-MORPHOGENETIC PROTEIN, INDUCE AXIAL MESODERM, SIGNALING PATHWAYS, EARLY RESPONSE, BETA-CATENIN, WNT PATHWAY, EMBRYOS, GENE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/146202
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item