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Magnetic Actuation of Smooth Muscle Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles

Angelopoulos, I; (2015) Magnetic Actuation of Smooth Muscle Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles. Doctoral thesis , UCL (University College London). Green open access

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Abstract

Faecal incontinence (FI) is a debilitating disorder that affects a significant portion of the population. The research included in this thesis aimed to test the hypothesis that magnetic actuating of smooth muscle cells loaded with superparamagnetic iron oxide nanoparticles (SPION) can modify the cell phenotype, which could be used with as a future therapy. The research focused on exploring a novel method of magnetic actuation and assessing its effects on the phenotype and biocompatibility of human rectal smooth muscle cells (HRSMC). A 2D model was used to demonstrate the effects of SPION on HRSMC. Initially, the effect of incubating HRSMC with different concentrations of SPION (0, 31.25, 250 and 1000 μg/ml) for 24 hours was investigated. Transmission electron microscopy revealed that SPION were endocytosed by cells and became concentrated inside endosomes. Superconducting quantum interference device (SQUID) measurements showed that SPION loading was concentration dependent and also that saturation occurred for concentrations above 250 μg/ml. SPION loading of HRSMC led to inhibition of the gene expression of actin and calponin when incubated in differentiation medium, with or without magnetic actuation, suggesting SPION caused the cells to shift towards a more proliferative phenotype. Live cell imaging revealed actuation of SPION-loaded HRSMC with stronger magnets led to an observable movement of internalized SPION and the plasma membrane. The findings from this research indicate SPION is biocompatible and may alter the phenotype of HRSMC. Therefore, SPION may offer novel benefits for regenerating damaged muscle in the treatment of FI. Further investigation is needed to assess the effects of magnetic actuation on SPION loaded cells.

Type: Thesis (Doctoral)
Title: Magnetic Actuation of Smooth Muscle Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles
Open access status: An open access version is available from UCL Discovery
Language: English
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1461225
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