Yusuf, M;
Millas, AL;
Estandarte, AK;
Bhella, GK;
McKean, R;
Bittencourt, E;
Robinson, IK;
(2014)
Platinum blue staining of cells grown in electrospun scaffolds.
BioTechniques
, 57
(3)
pp. 137-141.
10.2144/000114206.
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Abstract
Fibroblast cells grown in electrospun polymer scaffolds were stained with platinum blue, a heavy metal stain, and imaged using scanning electron microscopy. Good contrast on the cells was achieved compared with samples that were gold sputter coated. The cell morphology could be clearly observed, and the cells could be distinguished from the scaffold fibers. Here we optimized the required concentration of platinum blue for imaging cells grown in scaffolds and show that a higher concentration causes platinum aggregation. Overall, platinum blue is a useful stain for imaging cells because of its enhanced contrast using scanning electron microscopy (SEM). In the future it would be useful to investigate cell growth and morphology using three-dimensional imaging methods.
| Type: | Article |
|---|---|
| Title: | Platinum blue staining of cells grown in electrospun scaffolds |
| Location: | England |
| DOI: | 10.2144/000114206 |
| Publisher version: | http://dx.doi.org/10.2144/000114206 |
| Language: | English |
| Keywords: | cells, electrospinning, platinum blue, scaffold, scanning electron microscopy, sputter coating, staining, Cell Culture Techniques, Cell Line, Coloring Agents, Electrochemical Techniques, Fibroblasts, Humans, Lactic Acid, Microscopy, Electron, Scanning, Platinum, Polyglycolic Acid, Tissue Scaffolds |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1458004 |
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